Rebeads® Epoxy Magnetic Beads series are long-arm epoxy-modified agarose magnetic beads.The surface is grafted with epoxy groups carrying a special 12-carbon long extended arm. These groups can efficiently bind biological ligands with amino, sulfhydryl or hydroxyl groups.

With low steric hindrance, the beads can be directly applied to the purification and detection of antibodies and proteins in downstream experiments.

Product Performance

1. Abundant binding sites: High amine content enhances specific binding with ligands.
2. No pre-activation required, ready for direct use; the coupling reaction can be completed within 1–2 hours.
3. Reactions can be carried out under neutral pH and normal temperature (37 °C), with a suitable pH range of 7.0–8.5.
4. Compatible with nucleic acid extractors to realize high-throughput and automated operation.

Applications

Ideal for coupling with primary amine-containing proteins or small molecules, supporting affinity purification of antibodies, antigens, and other biomolecules. Compared with conventional carboxyl or amino magnetic beads, epoxy magnetic beads feature simpler operation and milder coupling conditions.

Work Flow

Antibody Surface Coupling

Figure: Antibody labeling process of carboxyl magnetic beads vs epoxy magnetic beads

Epoxy magnetic beads show comparable overall performance with carboxyl magnetic beads; they achieve higher signal-to-noise ratio at low concentration levels. Meanwhile, epoxy magnetic beads feature simple and convenient labeling procedure with excellent batch consistency.

1. Shake thoroughly before use to obtain a uniform microsphere suspension.
2. The coupling buffer used shall not contain ammonia; otherwise, the coupling efficiency will be affected.
3. For blocking, select an appropriate buffer solution according to practical application requirements.
4. This product is for research use only.