Carboxyl Magnetic Beads

Rebeads Carboxyl Magnetic Beads (NBC305) are surface-modified with carboxyl groups, suitable for nucleic acid extraction, fragment size selection, protein modification, chemiluminescence immunoassay, etc. Featuring uniform and moderate particle size, excellent dispersibility and fast magnetic response, they perfectly meet diverse customer requirements.

• Uniform dispersion, short response time and easy handling
• High batch-to-batch repeatability ensures accurate and reliable experimental results
• Broad application prospects in traditional fields such as protein purification and immunoassay, with outstanding operability and stability

Table 1 Performance Comparison between Rebeads Carboxyl Magnetic Beads and Competitors

Figure 2. Performance Comparison Between Rebeads® Carboxyl Magnetic Beads and Competitor Products

The carboxyl group content of Rebeads® magnetic beads is approximately 1.67 times that of the imported reference beads. The larger particle size provides a greater surface area, enabling equivalent separation performance at lower bead usage with appropriate reagents, delivering distinct advantages in small fragment recovery. The beads also exhibit higher protein conjugation efficiency compared to imported counterparts.

Three batches of Rebeads® Carboxyl Magnetic Beads were tested for fragment selection capability to verify batch-to-batch stability. For each batch, 50 µL of the same sample was incubated with the corresponding volume of magnetic beads, followed by washing and elution steps. The purified products were then analyzed by gel electrophoresis to assess the integrity of bands within the target range.

Figure: Fragment Recovery Performance of Different Batches of Carboxyl Magnetic Beads

The results demonstrate that:

1. The DNA recovery rate and fragment selection range of in-house produced magnetic beads are comparable to those of the control beads;
2. The three batches of in-house produced beads show minimal batch-to-batch variation, indicating a stable manufacturing process.

1. Operations such as freezing, drying, and centrifugation may cause bead aggregation, making resuspension and dispersion difficult.
2. Always thoroughly vortex the product before use to ensure a homogeneous bead suspension.
3. It is recommended to use high-quality pipette tips and reaction tubes to prevent sample loss due to non-specific binding of beads or solution.