NanoCytex Cell Sorting Column

The sorting column is syringe-shaped and filled with hydrophilic-coated iron beads with large gaps between beads to ensure proper flow rate without causing cell damage. The sorting column itself has no magnetism. When mounted on a magnetic rack, the internal iron beads become magnetized, amplifying the effective magnetic field by approximately 10,000 times. During sorting, cells only need to couple with a small amount of magnetic beads to be captured by the magnetized iron beads. Therefore, there is no need for additional magnetic bead removal in downstream experiments. After removing the sorting column, use the matched pusher to elute magnetically labeled cells and obtain the target cells.

Application Scope

It is used for the sorting of human CD3+ T cells. By incubating cells with nano-scale CD3 sorting magnetic beads, the isolation of CD3+ T cells can be achieved

Human CD4+ T cells in peripheral blood mononuclear cells (PBMCs) were sorted using RuibeiXi sorting columns, and the purity of the target cells after sorting was analyzed.

Display of the sorting results of Rebeads sorting column

Before sorting, the purity of CD4+ cells in human PBMCs was 35.1%, and the purity of CD4+ cells reached 97.7% after sorting.

Human CD4+ T cells were isolated from human PBMCs using Rebeads sorting columns, and the purity of the sorted target cells was analyzed.

Figure: Flow cytometric analysis of detection results after sorting human PBMCs

Based on flow cytometry results, the purity of sorted CD4+ cells increased from 39.8% to 99.6%. It demonstrates that Rebeads® NanoCytex CD4 magnetic beads deliver high sorting efficiency with simple, fast operation.

Figure 2 Comparison of sorting results with different sorting columns

The viability and recovery rate of CD4+ cells isolated from human PBMCs using commercial CD4 cell sorting magnetic beads combined with Rebeads sorting columns are significantly higher than those of competitors’ products.

1. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.