NanoCytex Cell Sorting Column

NanoCytex LS Columns

The sorting column is syringe-shaped and filled with hydrophilic-coated iron beads with large gaps between beads to ensure proper flow rate without causing cell damage.The sorting column itself has no magnetism. When mounted on a magnetic rack, the internal iron beads become magnetized, amplifying the effective magnetic field by approximately 10,000 times.During sorting, cells only need to couple with a small amount of magnetic beads to be captured by the magnetized iron beads. Therefore, there is no need for additional magnetic bead removal in downstream experiments.After removing the sorting column, use the matched pusher to elute magnetically labeled cells and obtain the target cells.

Key Features

• Superior performance with purity over 95%;
• Wide sample compatibility, enabling direct capture of target cells;
• High biosafety: sterile, low endotoxin, and biodegradable magnetic beads.

Work Flow

## Application Case ### 1. Sorting Performance Example Human CD4+ T cells were isolated from human PBMCs using Rebeads sorting columns, and the purity of the sorted target cells was analyzed.## Application Case ### 1. Sorting Performance Example Human CD4+ T cells were isolated from human PBMCs using Rebeads sorting columns, and the purity of the sorted target cells was analyzed.

Application Case

1. Sorting Performance Example

Figure: Flow cytometric analysis of detection results after sorting human PBMCs

Based on flow cytometry results, the purity of sorted CD4+ cells increased from 39.8% to 99.6%. It demonstrates that Rebeads® NanoCytex CD4 magnetic beads deliver high sorting efficiency with simple, fast operation.

2. Market Product Comparison Example