Rebeads® Protein L Magnetic Beads are monodisperse superparamagnetic beads with high-quality recombinant Protein L (purity > 95%) covalently immobilized on the surface.
Compared with Protein A or Protein G magnetic beads, Protein L beads bind to a wider spectrum of Ig classes and subclasses from humans, mice and rats, allowing more flexible antibody purification.
These beads also enable rapid and efficient one-step purification of antibodies from serum, ascites, plasma and cell culture supernatant of multiple species.
Product Performance
Selectivity: Immobilized Protein L is suitable for the selective purification of human and mouse antibodies with κ light chains.
Low non-specific binding: Stable, pre-blocked magnetic beads enable clean antibody purification.
Versatility: Compatible with manual and automated workflows (e.g., Rebeads® multifunctional purification system).
Applications
Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads.
Protein IP and co-IP assays.
Purification of ScFv and Fab fragments containing κ light chains.
Technical Datasheet
| Name |
Rebeads® Magrose Protein L |
Rebeads® Mag Protein L |
| Art.No |
NBG706 |
NBA603 |
| Ligand |
Protein L |
NBG705 |
| Shell |
Agarose |
Polymer |
| Particle size |
10-37μm |
1μm |
| Concentration |
20% v/v |
10mg/mL |
| Magnetism |
Superparamagnetism |
| Specification |
5ml/50ml/100ml |
| Magnetism |
Ultra pure water；48 months |
| Applications |
Protein Assays & AnalysisProtein Purification & Isolation |
| Remarks |
For Research Use Only |
Work Flow
Application Cases
- High antibody binding capacity
Figure: Antibody purification with Rebeads® Protein L magnetic beads achieves a purity of over 95%.
Precautions
Do not centrifuge, dry or freeze the magnetic beads. These operations may cause bead aggregation and reduce binding activity.
This product should be used with magnetic separation equipment.
Store at 2–8°C for stable storage; shelf life is 2 years.
This product is for research use only.
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