Mag COOH 2800 Carboxyl Magnetic Beads Excellent Stability High Flexibility

Brief Introduction

Carboxyl magnetic beads are superparamagnetic microspheres functionalized with carboxyl groups on their surface. Their core characteristic lies in the versatility of the carboxyl group: it can be simply chemically activated (typically using carbodiimide catalysts like EDC/NHS) to form stable amide bonds with biological molecules (such as amines on proteins, antibodies, or nucleic acids), thereby achieving covalent coupling.

Simply put, carboxyl magnetic beads are like a "universal magnetic platform" or a "blank canvas." Users can immobilize various "functional brushes" (biological ligands) onto them according to their specific needs, thus customizing magnetic separation tools for particular targets.

Product Features

Strong Versatility: Can be coupled to any biological molecule containing primary amine groups, including antibodies, proteins, peptides, oligonucleotides, enzymes, etc., resulting in an extremely broad application range.

High Binding Capacity: Optimization of surface chemistry and particle size provides a large specific surface area, enabling high-load coupling and enhancing detection sensitivity.

High Coupling Efficiency: The chemical reaction is mature and efficient, and can proceed under mild aqueous conditions.

Excellent Stability: The covalent bond is strong; prepared capture beads can be stored long-term with stable performance.

Low Non-specific Adsorption: Optimized surface modification and blocking processes effectively reduce non-specific binding to non-target substances, yielding a high signal-to-noise ratio.

High Flexibility: Users can couple their required ligands themselves, meeting the needs of personalized research and product development.

Technical Info

Main Application Fields

Carboxyl magnetic beads are the cornerstone of customized bio-separation technology, and their applications cover almost all magnetic separation scenarios.

Immunoassays and Diagnostics
This is the largest application area. By coupling specific antibodies to carboxyl magnetic beads to create immunomagnetic beads, they are used for:

Chemiluminescence Immunoassays: Serving as the solid-phase carrier for capture and separation, widely used in fully automated chemiluminescence immunoanalyzers to detect tumor markers, hormones, infectious disease markers, etc.

Immunochromatography: Used as a label in fluorescent or magnetic immunochromatographic test strips to improve detection sensitivity.

Cell Sorting: Isolating target cells (e.g., CD4+ T cells, circulating tumor cells - CTCs) from blood or tissue suspensions by coupling antibodies against specific cell surface markers. Although more specialized cell sorting beads have special coatings, the principle is based on this.

Nucleic Acid Extraction and Purification
Although not directly involved in binding, carboxyl magnetic beads are an important precursor for preparing silanol magnetic beads. Surface chemistry can convert carboxyl groups into silanol groups used for DNA/RNA extraction. Additionally, they can be directly coupled with nucleic acid probes for capturing specific sequences.

Protein and Peptide Purification

Affinity Purification: Coupling specific antibodies or ligands (e.g., nickel ion chelators) for tag proteins (like GST, His tags) to purify recombinant proteins.

Co-immunoprecipitation: Coupling antibodies against a target protein to "pull down" the protein and its interaction complexes from cell lysates, studying protein-protein interactions.

Enzyme Immobilization: Fixing enzymes onto the beads facilitates recovery and reuse after catalytic reactions.

Food Safety and Environmental Monitoring

Rapid Pathogen Detection: Enriching and detecting trace microorganisms in food or water samples by coupling specific antibodies against pathogens like Salmonella and E. coli.

Toxin Detection: Using competitive immunoassays by coupling antigens for small molecules like aflatoxin and ochratoxin.

Drug Screening and Targeted Therapy

Target Fishing: Immobilizing drug target proteins on the beads to screen lead compounds from natural product libraries that can bind to them.

Magnetic Targeted Drug Delivery: Loading drugs onto carboxyl magnetic beads and guiding them to the lesion site via an external magnetic field for targeted therapy.

Summary

Thanks to their exceptional versatility, high binding capacity, and stable chemical properties, carboxyl magnetic beads have become a core platform material in the fields of bioconjugation and magnetic separation. They are the key component in numerous high-end immunoassay reagents, cell sorting kits, and nucleic acid extraction reagents. Playing an indispensable role from basic research to industrial applications, carboxyl magnetic beads are the "universal key" in the modern biotechnology toolbox.