Methylated DNA Extraction Magnetic Beads High Consistency For Epigenetic Research

Brief Introduction

Methylated DNA extraction magnetic beads are specially surface-modified, superparamagnetic, nano-scale microspheres. Their core design principle is based on 5-methylcytosine antibodies or methylated DNA-binding domain proteins, enabling them to specifically recognize and bind methylated fragments within genomic DNA (i.e., fragments where a methyl group is added to the 5th carbon atom of the cytosine in CpG dinucleotides).

Unlike traditional genomic DNA extraction beads (which primarily rely on the non-specific binding of silanol groups to the DNA backbone), methylated DNA extraction magnetic beads are designed to "enrich" methylated fragments from complex whole-genome DNA for subsequent in-depth analysis.

Product Features:

• High Specificity & Affinity

  • Specifically designed to target and bind methylated DNA, ensuring superior enrichment and reducing non-specific carryover.

• Exceptional Sensitivity

  • Capable of efficiently isolating methylated DNA from limited and challenging samples, including cell-free DNA (cfDNA), FFPE tissues, and low-input samples.

• Maintained DNA Integrity

  • Provides gentle extraction that preserves DNA strand integrity and methylation status, ensuring reliable results for bisulfite conversion and subsequent analysis.

• Broad Sample Compatibility

  • Optimized for use with various sample types, including blood, plasma, serum, tissues, and cells.

• Streamlined, Automation-Friendly Workflow

  • The magnetic beads are compatible with manual protocols and automated high-throughput nucleic acid extraction systems, facilitating a rapid and consistent workflow without the need for organic solvents.

• High Consistency & Reproducibility

  • Manufactured with uniform size distribution and superior dispersion, guaranteeing batch-to-batch reproducibility and robust experimental performance.

Main Application Fields

Methylated DNA extraction magnetic beads are a powerful tool for epigenetic research, with main applications including:

Basic Scientific Research

Genomic Imprinting: Studying parent-of-origin-specific methylation patterns.

X Chromosome Inactivation: Exploring the epigenetic mechanism behind the silencing of one X chromosome in female cells.​

Embryonic Development and Cell Differentiation: Tracking dynamic changes in whole-genome methylation patterns during development.​

Tumorigenesis Mechanisms: Investigating the roles of oncogene hypomethylation and tumor suppressor gene hypermethylation in cancer development and progression.

Biomarker Development and Precision Medicine

Early Cancer Screening and Diagnosis: Enables non-invasive early diagnosis of cancers like lung cancer, colorectal cancer, and liver cancer by

detecting specific methylation markers in circulating tumor DNA from blood (liquid biopsy).​

Disease Subtyping and Prognosis Assessment: Specific methylation profiles are closely related to tumor malignancy, treatment efficacy, and patient survival.​

Medication Guidance: The efficacy of certain drugs is related to the methylation status of specific genes.​

Agriculture and Biotechnology

Crop Breeding: Researching epigenetic variations induced by environmental stresses (e.g., drought, salinity) to aid in selecting superior traits.​

Livestock Breeding: Studying methylation markers associated with economic traits (e.g., meat yield, milk production).

Technical Info

Summary

Methylated DNA extraction magnetic beads are an innovative tool for targeted methylated DNA enrichment. Their high specificity, sensitivity, and operational convenience have significantly advanced epigenetics, particularly in the fields of disease biomarker discovery and precision medicine. With continuous advancements in antibody technology and bead craftsmanship, their performance and application scope will further expand, making them an indispensable tool in life science and medical research.

Key Precautions for Use

• Prevent Agglomeration: Avoid freezing, drying, and centrifugation, which can cause irreversible bead clumping.
• Ensure Proper Resuspension: Vortex thoroughly immediately before use to maintain homogeneity.
• Note on Color: Slight color variation is normal under correct storage and does not indicate a change in performance.