Rebeads NanoCytex™ Cell Sorting Column

The NanoCytex Sorting Column features a syringe-like design. It is filled with uniform hydrophilic-coated iron microspheres with ample inter-sphere gaps to sustain flow rate and protect cells.
The column has no inherent magnetism. Once placed on a magnetic rack, the embedded iron microspheres are magnetized, boosting the effective magnetic field roughly 10,000 times.
Only a low quantity of conjugated magnetic beads is needed for cell capture, so bead removal is unnecessary for downstream experiments.
Target cells labelled with magnetic beads can be recovered using the supplied plunger after taking the column off the magnetic stand.

• Tolerance: Large internal space, low clogging risk; compatible with multiple magnetic beads for wide applications.
• High Performance: High purity, recovery and viability; cell purity ≥95%.
• Biosafety: Sterile, low endotoxin; biocompatible hydrophilic coating, cell-friendly.

Technical Specifications

Applications

1. Sorting Performance Example Isolation of CD4⁺ T cells from human PBMCs with Rebeads M Columns for post-sorting purity analysis.

Figure. Sorting results of Rebeads sorting column

Before sorting, the purity of CD4⁺ cells in human PBMCs was 35.1%. After sorting, the purity of CD3⁺ cells reached 97.7%.

2. Comparison with commercial products Rebeads M Columns are suitable for the isolation of human CD3⁺ T cells. Combined with NanoCytex CD3 magnetic beads, they enable gentle and efficient enrichment of high-purity and high-viability CD3⁺ T cells from complex samples.

Figure: Sorting result comparison of different columns

CD3⁺ T cells isolated from human PBMCs with commercial CD3 beads and Rebeads columns achieve much higher viability and yield than competing products.

Precautions

1. Research use only; not for diagnosis or therapy.
2. Aseptic handling is required for all steps.
3. Clumpy cell suspension or buffer can block the column and reduce sorting efficiency.
4. Not compatible with particles >30 μm. Filter and resuspend cells before sorting.