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Product Details:
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| Highlight: | Plasmid DNA Extraction Kit Centrifugation Free,Plasmid DNA purification Kit Centrifugation Free,Double Mag Beads Plasmid Extraction Kit |
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This kit adopts specially modified silica nano-magnetic microspheres. Under the action of a unique buffer system and external magnetic field, it can extract trace DNA from complex biological samples and purify it into high-purity DNA. It is compatible with our self-developed Mycoplasma DNA Detection Kit (Fluorescent Probe Method), for qualitative detection of mycoplasma contamination in master cell banks, working cell banks, virus seed batches and clinical therapeutic cells.
High Sensitivity: Limit of detection up to 10 CFU/mL; verified by parallel comparison with culture method, its sensitivity is equivalent to or superior to the culture method.
Fast & Convenient: The entire extraction process can be completed within 1 hour when matched with a nucleic acid extractor.
Automation Compatible: Suitable for high-throughput testing; supports both manual extraction and automated extraction with fully automatic nucleic acid extractors.
Wide Compatibility: Applicable for mycoplasma DNA extraction from various biological products; also compatible with detection kits from other manufacturers.
Qualitative detection of mycoplasma contamination in master cell banks, working cell banks, virus seed batches and cell products.
| Reagent Name | 50 Reactions Specification | Quantity | Storage Condition |
|---|---|---|---|
| Lysis Solution 1 | 1.4 mL | 2 Tubes | 2-8℃ |
| Lysis Solution | 14 mL | 1 Bottle | Room Temperature |
| Magnetic Bead Suspension | 5.5 mL | 1 Bottle | Room Temperature |
| Washing Solution A | 11 mL | 1 Bottle | Room Temperature |
| Washing Solution B | 11 mL | 1 Bottle | Room Temperature |
| Elution Buffer | 6 mL | 1 Bottle | Room Temperature |
| Diluent | 11 mL | 1 Bottle | Room Temperature |
Do not freeze or centrifuge the magnetic bead suspension before use to avoid damaging magnetic beads; mix thoroughly before application.
Lysis & Binding Solution may form white crystals below 10℃. If precipitation occurs, dissolve completely in a 37℃ water bath before use.
Perform subsequent DNA detection on the same day after sample purification to ensure accurate test results.
Read the kit instructions carefully and follow the operating procedures strictly.
Contact Person: Miss. Yammy
Tel: +8617712128819