Magnetic Bead-Based Total RNA Extraction Kit for Cells, Tissues and Blood

This Magnetic Bead-Based Total RNA Extraction Kit is designed for high-throughput isolation of high-quality total RNA from cell cultures, animal tissue samples and fresh whole blood.

It features high efficiency, speed and ease of use, and is fully compatible with automated nucleic acid extraction systems. Extraction of 96 samples can be completed within only 40 minutes.

RNA isolated with this kit delivers superior purity with negligible protein and genomic DNA contamination.

Figure 1. Workflow Diagram

• High-quality yield: Extracted RNA features high purity and intact integrity, directly applicable to PCR, NGS and various downstream experiments.
• Wide sample compatibility: Suitable for a variety of cells, animal tissues, fresh blood and body fluid samples.
• Safe & eco-friendly: No need for organic reagents such as phenol and chloroform, reducing harm to operators and environmental pollution.
• Fast & efficient: RNA extraction and purification can be completed within less than 1 hour, greatly improving experimental efficiency.
• High-throughput & automated: Meets large-scale experimental demands, reduces manual operation errors and ensures high reproducibility of results.

Total RNA was extracted from 8 groups of Jurkat cell samples using this kit. Results from agarose gel electrophoresis, capillary electrophoresis and qRT-PCR verify that the RNA extracted by Rebeads cellular RNA extraction kit presents high purity and excellent reproducibility.

This product was used to extract total RNA from various mouse tissues. Primers were designed targeting the β-actin housekeeping gene. PCR amplification was performed using the extracted RNA products and the cDNA generated by reverse transcription of the RNA as templates. The obtained PCR products were verified by agarose gel electrophoresis and qRT-PCR.

Using this kit to extract total RNA from different volumes of fresh blood, the extraction performance was evaluated by agarose gel electrophoresis (A), yield, purity and RQN value (C), as well as qRT‑PCR analysis (B). The results showed that the RNA extracted with this product exhibited good yield, purity and band integrity, with essentially no gDNA contamination, making it suitable for downstream applications such as library construction and qRT‑PCR.

When using this product with other reagents, follow the specific experimental protocols provided.

Some buffers in this kit contain irritants; wear appropriate personal protective equipment during handling.

Magnetic bead carryover may occur during elution. Avoid aspirating beads when collecting the eluted sample.

For research use only. Not for diagnostic use.