In this video, we demonstrate how NHS magnetic beads enable direct covalent coupling of proteins, antibodies, and other primary amine-containing biomolecules without pre-activation. Watch a step-by-step walkthrough of the coupling workflow, see real coupling efficiency data for an anti-AFM1 monoclonal antibody, and learn practical tips for buffer preparation, bead handling, and storage to achieve stable, high-efficiency ligand immobilization for affinity purification and immunoassay applications.