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| Highlight: | Large volume Plasmid Extraction Kit,Endotoxin free Plasmid Extraction Kit,plasmid purification kit Simple Operation |
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【Intended Use】
This product is suitable for extracting plasmid DNA from 50-100mL bacterial cultures. Its unique reagent system can effectively remove endotoxins and impurities such as proteins. The extracted plasmid DNA can be used for various molecular biology experiments like transfection, sequencing, restriction digestion, ligation, and transformation.
【Detection Principle】
DNA binds to the surface of silicon-based Magbeads under high salt conditions. After multiple washes to remove impurities such as proteins.The DNA can be eluted in high-purity under low salt conditions.
【Main Components】
| Components | SP104-10 | SP104-20 | SP104-50 |
| Buffer1 | 53mL | 105mL | 260mL |
| Buffer2 | 53mL | 105mL | 260mL |
| Buffer3 | 53mL | 105mL | 260mL |
| Buffer ETR | 30mL | 60mL | 150mL |
| Magnetic Beads for Endotoxin Removing | 21mL | 42mL | 105mL |
| Magnetic Beads for Nucleic Acid Extraction | 1.1mL×2 | 4.4mL | 11mL |
| Wash buffer |
45mL (add 45mL absolute ethanol) |
90mL (add 90mL absolute ethanol) |
220mL (add 220mL absolute ethanol) |
| Elution Buffer | 90mL | 180mL | 450mL |
| RNaseA | 0.53mL | 1.05mL | 1.3mL×2 |
【Storage Conditions and Shelf Life】
Buffer ETR and Magnetic beads should be stored at 2-8°C, RNase A at below 4°C, and other components at room temperature (15-25°C).
Acceptable conditions for shipment (within 14 days): 4-37℃;
Shelf life: 1 year at recommended storage condition
Contact Person: Mr. Ryan
Tel: +8618114465067