This product takes advantage of the characteristic that DNA binds to and elutes from Magbeads under specific conditions. It purifies PCR products above 150 bp and effectively removes impurities such as primer dimers, dNTPs, inorganic salts and proteins. The kit features high recovery rate, simple operation and fast processing speed.
Product Performance
High recovery rate and high purity, fully meeting the requirements of downstream experimental applications.
Wide recoverable DNA fragment range: 150 bp–15 Kb.
Simple and fast operation, no repeated centrifugation required.
Compatible with manual operation and automated workflows.
Application Scope
Suitable for the purification of conventional specific PCR products and PCR products in NGS library construction.DNA fragments purified by this kit can be directly used for downstream molecular biology experiments, including sequencing, PCR template preparation, enzyme digestion, ligation, and probe labeling.
Technical Parameters
| Component |
SP401-10 |
SP401-50 |
SP401-100 |
SP401-150 |
| Binding Buffer |
10 mL |
50 mL |
100 mL |
150 mL |
| Magnetic Bead Suspension |
1.25 mL |
6.25 mL |
12.5 mL |
18.75 mL |
Workflow
Application Case
Recovery Range Verification
Figure 1
Lane 1: MarkerLane 2: Purified product obtained using this kit
Figure 2
Lane 1: MarkerLane 2: Purified product obtained using this kit
This kit can purify DNA fragments with a minimum size of 150 bp and a maximum size of 15 kb or even longer.
2. Recovery Rate Comparison with Domestic and Foreign Products
M: Marker;Lane 1, 5: Products before purification;Lane 2, 6: Purified products of our company’s kit;Lane 3, 7: Purified products from a domestic manufacturer;Lane 4, 8: Purified products from a foreign manufacturer.
The recovery rate of our product is comparable to peer products, while its purity is superior to others.
Notes
When used with other reagents, please follow the specific experimental instructions for operation.
Some buffers in this kit contain irritant substances; please take proper protective measures during handling.
Magnetic bead residue may remain after elution; avoid aspirating magnetic beads when transferring samples.
This product is for research use only.
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