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Product Details:
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| Highlight: | Efficient Genomic DNA Extraction Kit,Efficient Genomic DNA purification Kit,DNA Extraction Kit Safe Operation |
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This kit adopts the magnetic bead method to isolate and purify genomic DNA from 3×3 mm dried blood spot samples.Under high-salt conditions, DNA binds to the surface of silica-coated magnetic beads. Impure proteins and contaminants are removed through multiple washing steps, and target DNA is eluted under low-salt conditions.
This product is suitable for genomic DNA extraction from dried blood spots.
| Components | GP122-50 | GP122-100 | GP122-300 | GP122-500 |
|---|---|---|---|---|
| Lysis Solution | 26 mL | 55 mL | 165 mL | 260 mL |
| Washing Solution | 15 mL (Add 19.5 mL absolute ethanol) |
30 mL (Add 39 mL absolute ethanol) |
90 mL (Add 117 mL absolute ethanol) |
150 mL (Add 195 mL absolute ethanol) |
| Elution Buffer | 6 mL | 12 mL | 36 mL | 55 mL |
| Magnetic Bead Suspension | 1.05 mL | 2×1.05 mL | 6.5 mL | 10.5 mL |
| Proteinase K | 1.05 mL | 2×1.05 mL | 6.5 mL | 10.5 mL |
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Magnetic Bead-Based Genomic DNA Extraction from Dried Blood Spots — Comparison Test with Kits from Different Manufacturers
The same amount of dried blood spots (4 pieces of 3×3 mm), the same elution volume (50 μL), and the same gel loading volume (6 μL) were used for comparison among magnetic bead-based blood spot kits from different companies.
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Figure: Electrophoresis results of genomic DNA extracted from dried blood spots.Rebeads magnetic bead-based dried blood spot genomic DNA extraction kit shows higher yield than domestic Company A, B and C.
Before first use of a new kit, add the specified volume of absolute ethanol to the washing solution as labeled, and set aside for later use.
Do not freeze magnetic beads; mix the magnetic bead suspension thoroughly before use.
If RNase A or nucleic acid coprecipitant is used, store at -20 ℃, avoid repeated freezing and thawing; sub-packaging and frozen storage are recommended.
Contact Person: Miss. Yammy
Tel: +8617712128819