Rebeads® NHS Magnetic Beads from Rebeads are surface-modified with high-density NHS functional groups.
They can covalently conjugate with primary amine (-NH₂)-containing biomolecules (proteins, antibodies, peptides, etc.) to form stable amide bonds.
No pre-activation is required; conjugation is achieved by simple mixing.
The procedure is simple, efficient and mild.
These beads are widely applied in affinity purification, immunoassays, IP/Co-IP and other applications.
Product Description
| Product Name |
Rebeads® Mag NHS |
Rebeads® Magrose NHS |
Rebeads® Magrose NHS 65 |
| Matrix |
Polymer |
Agarose |
Agarose |
| Catalog No. |
NBH901 |
NBH912 |
NBH913 |
| Particle Size |
1 μm |
10–37 μm |
37–100 μm |
| Binding Capacity |
≥30 μg Rabbit IgG / mg beads |
≥30 mg Rabbit IgG / mL beads |
≥30 mg Rabbit IgG / mL beads |
| Bead Concentration |
10 mg/mL |
50% v/v |
50% v/v |
| Stability |
Stable storage at 2–8°C, shelf life: 1 year |
|
|
| Storage Buffer |
DMAC |
Anhydrous Isopropanol |
Anhydrous Isopropanol |
| Applications |
Protein purification, antibody purification, analytical detection (such as in vitro diagnostics, microbial detection, IP, Co‑IP, etc.) |
Product Advantages
✅ Simple Operation No pre‑activation required; covalent conjugation with biological ligands directly.
✅ Fast & Efficient High-efficiency conjugation completed within 1–2 h. Both conjugation efficiency and loading capacity are superior to carboxylated magnetic beads.
✅ Mild Reaction Conditions Conjugation can be performed at room temperature or 4 ℃, with a working pH range of 4–10.
✅ Stable Conjugation Forms stable amide bonds to prevent ligand shedding; low non-specific adsorption.
Application Directions
In vitro diagnostics IP, Co‑IP, Microbial detection
Antibody purification, Protein purification, Macromolecule conjugation
Application Test
Conjugation Test of AFM1 Monoclonal Antibody on NHS Magnetic Beads (Determined by Bradford Protein Assay)
| AFM1 mAb Loading Amount (μg) |
Conjugated Amount (μg) |
Conjugation Efficiency (%) |
| 5.00 |
4.67 |
93.40 |
| 10.00 |
9.54 |
95.40 |
| 20.00 |
19.27 |
96.35 |
| 30.00 |
29.32 |
97.73 |
Note: Bead dosage: 100 μL; Conjugation system: 1 mL Coupling Buffer. As the antibody concentration increases, more antibodies are conjugated onto the beads, with conjugation efficiency higher than 97%.
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